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plasmid plko 1 puro scramble  (Addgene inc)


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    Structured Review

    Addgene inc plasmid plko 1 puro scramble
    Plasmid Plko 1 Puro Scramble, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1566 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko%2E1+scramble/pLKO%2E1+puro+(Plasmid+%238453)/pmc12978252-240-25-27
    Average 96 stars, based on 1566 article reviews
    plasmid plko 1 puro scramble - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: ΔNp63α drives serine synthesis to promote carboplatin resistance in NSCLC
    Article Snippet: The short hairpin RNAs (shRNAs) targeting human p63 or ATF4 was generated by inserting specific oligoes into a pLKO.1-puromycin lentiviral vector (10878, Addgene, Cambridge, MA, USA). .. The pLKO.1-scramble vector (#1864, Addgene) was used as a negative control vector and contained a scrambled shRNA (shC). ..

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer.
    Article Snippet: The HCC1806 (ATCC CRL- 2335), HCC38 (ATCC CRL- 2314), and ZR- 75- 1 (ATCC CRL- 1500) human breast cancer cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS, penicillin (100 U/ ml), streptomycin (100 μg/ml), and amphotericin B (1 μg/ml). .. Three shRNA pLKO.1 plasmids each against RBM4B (TRCN0000158566, TRCN0000159492, and TRCN0000159895) and SAMDA4B (TRCN0000433715, TRCN0000412300, and TRCN0000005449) were purchased ready made from Millipore with the plasmid pLKO.1- Puro- Scramble (Addgene, #162011) acting as negative control. .. These plasmids were then cotransfected with lentiviral packaging plasmids pCMV- R8.91 (Addgene, #202687) and pMD2.G (Addgene, #12259) into HEK293T cells using the TransIT- Lenti Transfection Reagent (MirusBio, MIR 6600) according to the manufacturer’s instructions for lentivirus production.

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer
    Article Snippet: The shRNAs shRBMS3-1 (TRCN0000152525) and shRBMS3-2 (TRCN0000152012) were cloned into plasmid pLKO.1-Puro (Addgene, #8453). .. Three shRNA pLKO.1 plasmids each against RBM4B (TRCN0000158566, TRCN0000159492, and TRCN0000159895) and SAMDA4B (TRCN0000433715, TRCN0000412300, and TRCN0000005449) were purchased ready made from Millipore with the plasmid pLKO.1-Puro-Scramble (Addgene, #162011) acting as negative control. .. These plasmids were then cotransfected with lentiviral packaging plasmids pCMV-R8.91 (Addgene, #202687) and pMD2.G (Addgene, #12259) into HEK293T cells using the TransIT-Lenti Transfection Reagent (MirusBio, MIR 6600) according to the manufacturer’s instructions for lentivirus production.

    Article Title: ΔNp63α drives serine synthesis to promote carboplatin resistance in NSCLC.
    Article Snippet: The short hairpin RNAs (shRNAs) targeting human p63 or ATF4 was generated by inserting specific oligoes into a pLKO.1-puromycin lentiviral vector (10878, Addgene, Cambridge, MA, USA). .. The pLKO.1-scramble vector (#1864, Addgene) was used as a negative control vector and contained a scrambled shRNA (shC). ..

    Negative Control:

    Article Title: ΔNp63α drives serine synthesis to promote carboplatin resistance in NSCLC
    Article Snippet: The short hairpin RNAs (shRNAs) targeting human p63 or ATF4 was generated by inserting specific oligoes into a pLKO.1-puromycin lentiviral vector (10878, Addgene, Cambridge, MA, USA). .. The pLKO.1-scramble vector (#1864, Addgene) was used as a negative control vector and contained a scrambled shRNA (shC). ..

    Article Title: Endogenous retroviral elements LTR8B and MER65 rewire PSG9 regulation to control trophoblast syncytialization and pre-eclampsia risk
    Article Snippet: .. The pLKO.1-scramble shRNA (#1864 Addgene, a gift from David Sabatini) was used as a negative control. .. Cell lysis was performed at 4 °C for 2 h using a lysis buffer containing 50 mM Tris–HCl (pH 8.0), 100 mM NaCl, 5% glycerol, 10 mM EDTA, 1% NP-40, and an appropriate amount of protease inhibitors (#A32955, Thermo Fisher Scientific).

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer.
    Article Snippet: The HCC1806 (ATCC CRL- 2335), HCC38 (ATCC CRL- 2314), and ZR- 75- 1 (ATCC CRL- 1500) human breast cancer cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS, penicillin (100 U/ ml), streptomycin (100 μg/ml), and amphotericin B (1 μg/ml). .. Three shRNA pLKO.1 plasmids each against RBM4B (TRCN0000158566, TRCN0000159492, and TRCN0000159895) and SAMDA4B (TRCN0000433715, TRCN0000412300, and TRCN0000005449) were purchased ready made from Millipore with the plasmid pLKO.1- Puro- Scramble (Addgene, #162011) acting as negative control. .. These plasmids were then cotransfected with lentiviral packaging plasmids pCMV- R8.91 (Addgene, #202687) and pMD2.G (Addgene, #12259) into HEK293T cells using the TransIT- Lenti Transfection Reagent (MirusBio, MIR 6600) according to the manufacturer’s instructions for lentivirus production.

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer
    Article Snippet: The shRNAs shRBMS3-1 (TRCN0000152525) and shRBMS3-2 (TRCN0000152012) were cloned into plasmid pLKO.1-Puro (Addgene, #8453). .. Three shRNA pLKO.1 plasmids each against RBM4B (TRCN0000158566, TRCN0000159492, and TRCN0000159895) and SAMDA4B (TRCN0000433715, TRCN0000412300, and TRCN0000005449) were purchased ready made from Millipore with the plasmid pLKO.1-Puro-Scramble (Addgene, #162011) acting as negative control. .. These plasmids were then cotransfected with lentiviral packaging plasmids pCMV-R8.91 (Addgene, #202687) and pMD2.G (Addgene, #12259) into HEK293T cells using the TransIT-Lenti Transfection Reagent (MirusBio, MIR 6600) according to the manufacturer’s instructions for lentivirus production.

    Article Title: ΔNp63α drives serine synthesis to promote carboplatin resistance in NSCLC.
    Article Snippet: The short hairpin RNAs (shRNAs) targeting human p63 or ATF4 was generated by inserting specific oligoes into a pLKO.1-puromycin lentiviral vector (10878, Addgene, Cambridge, MA, USA). .. The pLKO.1-scramble vector (#1864, Addgene) was used as a negative control vector and contained a scrambled shRNA (shC). ..

    shRNA:

    Article Title: ΔNp63α drives serine synthesis to promote carboplatin resistance in NSCLC
    Article Snippet: The short hairpin RNAs (shRNAs) targeting human p63 or ATF4 was generated by inserting specific oligoes into a pLKO.1-puromycin lentiviral vector (10878, Addgene, Cambridge, MA, USA). .. The pLKO.1-scramble vector (#1864, Addgene) was used as a negative control vector and contained a scrambled shRNA (shC). ..

    Article Title: Endogenous retroviral elements LTR8B and MER65 rewire PSG9 regulation to control trophoblast syncytialization and pre-eclampsia risk
    Article Snippet: .. The pLKO.1-scramble shRNA (#1864 Addgene, a gift from David Sabatini) was used as a negative control. .. Cell lysis was performed at 4 °C for 2 h using a lysis buffer containing 50 mM Tris–HCl (pH 8.0), 100 mM NaCl, 5% glycerol, 10 mM EDTA, 1% NP-40, and an appropriate amount of protease inhibitors (#A32955, Thermo Fisher Scientific).

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer.
    Article Snippet: The HCC1806 (ATCC CRL- 2335), HCC38 (ATCC CRL- 2314), and ZR- 75- 1 (ATCC CRL- 1500) human breast cancer cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS, penicillin (100 U/ ml), streptomycin (100 μg/ml), and amphotericin B (1 μg/ml). .. Three shRNA pLKO.1 plasmids each against RBM4B (TRCN0000158566, TRCN0000159492, and TRCN0000159895) and SAMDA4B (TRCN0000433715, TRCN0000412300, and TRCN0000005449) were purchased ready made from Millipore with the plasmid pLKO.1- Puro- Scramble (Addgene, #162011) acting as negative control. .. These plasmids were then cotransfected with lentiviral packaging plasmids pCMV- R8.91 (Addgene, #202687) and pMD2.G (Addgene, #12259) into HEK293T cells using the TransIT- Lenti Transfection Reagent (MirusBio, MIR 6600) according to the manufacturer’s instructions for lentivirus production.

    Article Title: Endogenous retroviral elements LTR8B and MER65 regulate the PSG9 locus that promotes trophoblast syncytialization: Insights into placental evolution and pre-eclampsia pathology
    Article Snippet: .. The pLKO.1-scramble shRNA (#1864 Addgene). .. Total RNA was isolated from tissues (homogenized by ceramic beads) and cells using QIAzol lysis reagent and Qiagen RNeasy mini kit (including on-column DNAase I digestion) (Qiagen) according to the manufacturer’s protocol.

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer
    Article Snippet: The shRNAs shRBMS3-1 (TRCN0000152525) and shRBMS3-2 (TRCN0000152012) were cloned into plasmid pLKO.1-Puro (Addgene, #8453). .. Three shRNA pLKO.1 plasmids each against RBM4B (TRCN0000158566, TRCN0000159492, and TRCN0000159895) and SAMDA4B (TRCN0000433715, TRCN0000412300, and TRCN0000005449) were purchased ready made from Millipore with the plasmid pLKO.1-Puro-Scramble (Addgene, #162011) acting as negative control. .. These plasmids were then cotransfected with lentiviral packaging plasmids pCMV-R8.91 (Addgene, #202687) and pMD2.G (Addgene, #12259) into HEK293T cells using the TransIT-Lenti Transfection Reagent (MirusBio, MIR 6600) according to the manufacturer’s instructions for lentivirus production.

    Article Title: ΔNp63α drives serine synthesis to promote carboplatin resistance in NSCLC.
    Article Snippet: The short hairpin RNAs (shRNAs) targeting human p63 or ATF4 was generated by inserting specific oligoes into a pLKO.1-puromycin lentiviral vector (10878, Addgene, Cambridge, MA, USA). .. The pLKO.1-scramble vector (#1864, Addgene) was used as a negative control vector and contained a scrambled shRNA (shC). ..


    other:

    Article Title: A neuron subtype-specific role of MEK-ERK signaling in axon survival via transcriptional regulation of Nmnat2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides PCR primers This study See Table S1 qPCR Nmnat2 forward primer: ATGACACCTGTGATCGGACA This study N/A qPCR Nmnat2 reverse primer: GAGGCTTTCTCCCACCTTTC This study N/A qPCR Gapdh forward primer: GTGTTCCTACCCCCAATGTG This study N/A qPCR Gapdh reverse primer: CCTGCTTCACCACCTTCTTG This study N/A Recombinant DNA Plasmid: psPAX2 Addgene RRID: Addgene_12260 Plasmid: pMD2.G Addgene RRID: Addgene_12259 Plasmid: pLenti-hSyn-Mek1-HA This study N/A Plasmid: pLenti-hSyn-Mkp1-Flag This study N/A Plasmid: pLenti-hSyn-Nmnat2-Flag This study N/A Plasmid: pLenti-hSyn-Creb-HA This study N/A Plasmid: pLenti-hSyn-Renilla This study N/A Plasmid: pLenti-hSyn-Mek1 CA -HA This study N/A Plasmid: pLenti-hSyn-Creb DIEDML -HA This study N/A Plasmid: pLenti-hSyn-Braf CA -HA This study N/A Plasmid: pLenti-Nmnat2-Firefly This study N/A Plasmid: pLKO.1 Addgene RRID: Addgene_10878 Plasmid: pLKO.1-shScramble Addgene RRID: Addgene_1864 Plasmid: pLKO.1-shMek1 This study See Table S2 Plasmid: pLKO.1-shMek2 This study See Table S2 Plasmid: pLKO.1-shErk1 This study See Table S2 Plasmid: pLKO.1-shErk2 This study See Table S2 Plasmid: pLKO.1-shNmnat2 This study See Table S2 Plasmid: pLKO.1-shCreb This study See Table S2 Software and algorithms Adobe Photoshop Adobe http://www.adobe.com/products/ photoshop.html GraphPad Prism GraphPad http://www.graphpad.com/ ImageJ NIH https://imagej.net/ Leica Application Suite X Leica https://www.leica-microsystems.com/ products/microscope-software/ details/product/leica-las-x-ls/ 16 Cell Reports 45, 116931, February 24, 2026 (Invitrogen, 35050061), penicillin streptomycin (Invitrogen, 10378016) and 40 ng/mL mouse NGF (Gibco, 13257-019) was gently added to each well.



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